Do you know what the Profiler calibration exactly is and what it is good for? Are you aware of the differences in your application behavior when being profiled? Do you know that some of application methods won't appear in collected results? And are you absolutely sure that you are able to get as much accurate results from the Profiler as possible?
If you are not absolutely sure about anything of the above, take a quick look at Profiling Tips in Profiler docs. You may find it a really useful reading.